A few days ago, the editor introduced GE's Superdex 75 Increase chromatography column, which is mainly used for the purification and analysis of recombinant protein molecules, as well as the Superose 6 Increase chromatography column designed specifically for the purification of large proteins and complexes. Today, we would like to introduce the third member of the "Increase Family" three swordsmen - Superdex 200 Increase chromatography column.
Superdex 200 Increase chromatographic column is pre loaded with a new generation of high flow rate agarose filler similar to Superose 6 Increase. The average particle size has reached an unprecedented 8.6 μ m, but it has higher mechanical strength, which meets the requirements of gel filtration chromatography and molecular exclusion chromatography for stronger column efficiency and higher resolution.
Superdex 200 Increase provides mild separation conditions, specifically designed for semi preparation and high-performance analysis and purification of proteins, DNA fragments, and other small molecules. It can maximize the integrity and activity of proteins, and enhance the results of subsequent protein structure and function research.
Superdex 200 Increase has excellent alkali resistance, greatly reducing the risk of cross contamination during repeated use while extending the lifespan of the column.
The extremely low non-specific adsorption level of Superdex 200 Increase brings higher sample recovery rates, especially suitable for the study of precious samples.
·Wide separation range: 10kDa-600kDa, covering the vast majority of research hotspots;
·Higher resolution: Compared to Superdex 200, the particle size has been reduced from 13 μ m to 8.6 μ m, resulting in a 60% increase in resolution;
·Faster running speed: Compared with Superdex 200, the running time is reduced by 50% at the same resolution;
·Stronger resistance to alkaline washing: able to withstand up to 1M NaOH, effectively preventing cross contamination, especially suitable for platform sharing;
·More versatile: equally excellent in sample purification and SEC analysis.
Maintain the same resolution while greatly saving runtime
The flow rate remains the same, but the resolution is significantly improved
Application orientation of the new generation of gel filtration platform "Increase family" three swordsmen
